The Complexities of Human Mitochondrial Inner-membrane Protein Translocases in Maintenance of Organeller Function

Abstract

Human mitochondria require ~1500 proteins for its constitutive functions. However, the mitochondrial genome is gene deficient and most of the mitochondrial proteome is nuclear encoded and transported into the organelle. Import of majority of mitochondrial proteome is mediated by presequence pathway and requires functional interplay of a subset of molecular chaperones, along with accessory factors. Although detailed analysis has been carried out in yeast system, our understanding of human mitochondrial proteome maintenance has been severely limited by absence of significant structural and functional information on the organization TIM23 complex in human. Recent studies have shown presence of multiple presequence translocases in human mitochondria that show considerable diversity in their substrate and physiological specificity. The core organization and inter-molecular interactions between these machineries seems to be evolutionary conserved. These translocases have principally diversified due to divergence of Hsp70 co-chaperones and channel component Tim17. Another important feature associated with human mitochondrial translocases, which in most cases absent in lower eukaryotes, is multifunctionality. Other than housekeeping protein import, human mitochondrial translocases have gained secondary functions, where they are involved in ROS sensing and regulation of redox balance, modulation of cellular sensitivity to xenobiotic drugs, maintenance to mitochondrial DNA, assembly of respiratory complexes and import of non-canonical mitochondrial substrates. The origin of multifunctionality is indicative towards the possibility of functional connections between mitochondrial protein import and regulation of cellular pathways; and hence, opens up new avenues of future research.