An evaluation of in-vitro potential of novel Lactobacillus paraplantarum KM1 (KX671558) strain isolated from milk
Keywords:
Probiotics, Lactobacillus paraplantarum KM1, autoaggregation, antimicrobial activity, antibiotics sensitivity
Abstract
Present study was carried out to evaluate probiotic potential of novel bacterial strain i.e. L. paraplantarum KM1 (accession number KX671558) isolated 1st time from pooled milk of Himachal Pradesh. L. paraplantarum KM1 exhibited highest viable counts at low acid value (i.e. at pH 2.0 and 3.0), bile tolerance (concentration 0.3%), autoaggregation (36.6%), co-aggregation with pathogenic bacteria (20.0%), cell surface hydrophobicity against O-xylene (95%), antimicrobial activity against various food borne pathogens (inhibition = 100%) and antibiotic sensitivity test which comes out to be sensitive against most of the antibiotics used. These all desirable attributes turn this strain a robust probiotic.
References
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Sanlibaba P and Cakmak G A (2016) Exopolysaccharides production by lactic acid bacteria Applied Microbiology: Open Access 2: 2
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Orłowski A, and Bielecka M (2006) Preliminary characteristics of lactobacillus and bifidobacterium strains as probiotic candidates Polish J Food Nutr Sci 56(3) 269–275
Ouwehand A C, Kirjavainen P V, Gronlund M M, Isolauri E and Salminen S J (2002) Adhesion of probiotic micro-organisms to intestinal mucus International Diary Journal 9 623-630
Puniya M, Kumar RM, Panwar H, Kumar N, Ramneek and Kumar AP (2016) Screening of lactic acid bacteria of different origin for their probiotic potential Food Processing and Technology 7 1
Rosenberg M, Gutnick D and Rosenberg E (1980) Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydrophobicity FEMS Microbiology Letters 9 29-33
Ruas-Madiedo P, Gueimonde M and Margolles A (2006) Exopolysaccharides produced by probiotic strains modify the adhesion of probiotics and enteropathogens to human intestinal mucus Food Protection 69 2011-2015
Sanderson I R and Walker W A (1993) Uptake and transport of macromolecules by the intestine: possible role in clinical disorders (an update) Gastroenterology 104 622-639
Singh T P, Malik R K, Kaur G and Renuka (2014) Safety assessment and evaluation of probiotic potential of Lactobacillus reuteri strains under in vitro conditions International Journal of Current Microbiology and Applied Science 3(2) 335-348
Syal P and Vohra A (2013) Probiotic potential of yeasts isolated from traditional Indian fermented Foods International Journal of Microbiology Research 5(2) 390-398
Vaz-Velho M and Todorov S D (2011) Potential probiotic evaluation of bacteriocin producing Lactobacillus plantarum ST16PA isolated from Papaya (Carica Papaya). 12th ASEAN Food Conference, Bangkok, Thailand.
Xanthopoulos V, Litopoulou-Tzanetaki E and Tzanetakis, N (1997) In vitro study of lactobacillus species strains on bile tolerance and cholesterol removal. In: Lactic Acid Bacteria - Lactic 97. Caen: Presses universitaires de Caen.
Zhao Q, Liu Z, Xue Y, Wang J, Li H, Tang Q, Wang Y, Dong P, Xue C and Ds-echinoside A (2011) A new triterpene glycoside derived from sea cucumber, exhibits anti metastatic activity via the inhibition of NF-kB-dependent MMP-9 and VEGF expressions. Journal of Zhejiang University- Science 12 534-544
Barefoot S F and Klanhammer T R (1983) Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus Applied Environmental Microbiology 45 1808-181
Sanlibaba P and Cakmak G A (2016) Exopolysaccharides production by lactic acid bacteria Applied Microbiology: Open Access 2: 2
Amina Z, Noureddine S, Venkatesan A, Perumal V, Hichem B, Asma Z, Yamina M, Miloud H and Mebrouk K (2014) Characterization and potential probiotic attributes of Lactobacillus plantarum DU10 isolated from Algerian raw Camel milk Biotechnology 13(6) 282-288
Arief I I, Jenie B S L, Astawan M, Fujiyama K and Witarto A B (2015) Identification and probiotic characteristics of lactic acid bacteria isolated from Indonasian local beef Asian Journal of Animal Sciences 9(1) 25-36
Bao Y, Zhang Y, Zhang Y, Lui and Wang S (2010) Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products Food Control 21 695-701
Begley M, Gahan C G and Hill C (2005) The interaction between bacteria and bile. FEMS Microbiology Review 4 625-51
Breed R S, Murray E G D and Smith N R (1957) Bergey’s Manual of Determinative Bacteriology, 7th ed., the Williams and Wilkins Co., Baltimore, American Society of Microbiology
CLSI (2007) Clinical and Laboratory Standards Institute Performance standards for antimicrobial susceptibility testing. 17th edition. Wayne, PA.
Collado M C, Meriluoto J and Salminen S (2007) Adhesion and aggregation properties of probiotic and pathogen strains European Food Research and Technology 222 1065-1073
De Vyust L, Foulquie M R and Revets H (2003) Screening for enterocins and detection of hemoolysin and vancomycin resistance in Enterococci of different origins International Journal of Food Microbiology 84 299-318
De Vuyst L, de Vin F, Vaningelgem F and Degeest B (2001) Recent developments in the biosynthesis and applications of heteropolysaccharides from lactic acid bacteria International Dairy Journal 11 687-707
Del Re B, Sgorbati B, Miglioli M and Palenzona D (2000) Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum Letters in Applied Microbiology 31 438-442
Delgado S, Leite A M O, Ruas-Madiedo P and Mayo B (2015) Probiotic and technological properties of Lactobacillus spp. strains from the human stomach in the search for potential candidates against gastric microbial dysbiosis Frontiers in Microbiology 5(766) 1-8
Fossi BT, Ekue NB, Nchanji GT, Ngah BG, Anyangwe IA and Wanji S (2015) Probiotic properties of lactic acid bacteria isolated from fermented sap of palm tree (Elaeis guineensis) Journal of Microbiology and Antimicrobials 7(5) 42-52
Fuller R (1989) Probiotics in man and animals Journal of Applied Bacteriology 66(5) 365-378
Gautam N and Sharma N (2014) Quality attributes of novel cereal based probiotic products prepared by using food grade lactic acid bacteria Indian Journal of Traditional Knowledge 13 525-530
Gotcheva V, Hristozova E, Hristozova T, Guo M, Roshko¬va Z and Angelov A (2002) Assessment of potential probiotic properties of lactic acid bacteria and yeast strains. Food Biotech¬nology 16 211-225
Gupta H and Malik R K (2007) Incidence of virulence in bacteriocin producing enterococcal isolates Lait 87 587 601
Harrigan W F (1998) Laboratory methods in food microbiology Academic Press: New York
Hasegawa T, Minami M, Okamoto A, Tatsuno I, Isaka M and Ohta M (2010) Characterization of a virulence-associated and cell wall located DNase of Streptococcus pyogenes Microbiology 186 184-190
Hati S, Vij S, Mandal S, Yadav D and Vandna (2014) Evaluation of probiotic potentials of L. plantarum C6 isolated from cheese. International Journal of Dairy Science 67(5) 413-420
Havenaar R and Huis in’t Veld J H J (1992) Probiotics: a general view. In: Wood B J B, ed. The lactic acid bacteria in health and disease. London: Elsevier Applied Science 1 151–71
Holzapfel W H, Geizen R and Schillinger U (1995) Biological preservation of foods with reference to protective cultures, bacteriocins and food grade enzymes International Journal of Food Microbiology 24 343-362
Kimura H, Sashihara T, Matsusaki H, Sonomoto K and Ishizaki A (1998) Novel bacteriocin of Pediococcus sp. ISK-1 isolated from well – aged bed of fermented rice bran Annals of New York Academy of Science 864 345-348
Kos J, Suskovic J, Vukovic S, Simprag M, Frece J and Matosvic S (2003) Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92 B Journal of Applied Microbiology 94 981–987
Li Q, Liu X, Dong M, Zhou J and Wang Y (2015) Aggregation and adhesion abilities of 18 lactic acid bacteria strains isolated from traditional fermented food International Journal of Agricultural Policy and Research 3(2) 84-92
Licht T R and Wilcks A (2005) Conjugative gene transfer in the gastrointestinal environment. Advances in Applied Microbiology 58 77-95
Lindgren S E and Dobrogosz W J (1990) Antagonistic activities of lactic acid bacteria in food and feed fermentations FEMS Microbiology Reviews 7 149- 163
Martin F P, Wang Y, Sprenger N, Yap I K, Lundstedt T, Lek P, Rezzi S, Ramadan Z, van Bladeren P, Fay L B, Kochhar S, Lindon J C, Holmes E and Nicholson J K (2008) Probiotic modulation of symbiotic gut microbial-host metabolic interactions in a humanized microbiome mouse model Molecular and Systemic Biology 4 157
Orłowski A, and Bielecka M (2006) Preliminary characteristics of lactobacillus and bifidobacterium strains as probiotic candidates Polish J Food Nutr Sci 56(3) 269–275
Ouwehand A C, Kirjavainen P V, Gronlund M M, Isolauri E and Salminen S J (2002) Adhesion of probiotic micro-organisms to intestinal mucus International Diary Journal 9 623-630
Puniya M, Kumar RM, Panwar H, Kumar N, Ramneek and Kumar AP (2016) Screening of lactic acid bacteria of different origin for their probiotic potential Food Processing and Technology 7 1
Rosenberg M, Gutnick D and Rosenberg E (1980) Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydrophobicity FEMS Microbiology Letters 9 29-33
Ruas-Madiedo P, Gueimonde M and Margolles A (2006) Exopolysaccharides produced by probiotic strains modify the adhesion of probiotics and enteropathogens to human intestinal mucus Food Protection 69 2011-2015
Sanderson I R and Walker W A (1993) Uptake and transport of macromolecules by the intestine: possible role in clinical disorders (an update) Gastroenterology 104 622-639
Singh T P, Malik R K, Kaur G and Renuka (2014) Safety assessment and evaluation of probiotic potential of Lactobacillus reuteri strains under in vitro conditions International Journal of Current Microbiology and Applied Science 3(2) 335-348
Syal P and Vohra A (2013) Probiotic potential of yeasts isolated from traditional Indian fermented Foods International Journal of Microbiology Research 5(2) 390-398
Vaz-Velho M and Todorov S D (2011) Potential probiotic evaluation of bacteriocin producing Lactobacillus plantarum ST16PA isolated from Papaya (Carica Papaya). 12th ASEAN Food Conference, Bangkok, Thailand.
Xanthopoulos V, Litopoulou-Tzanetaki E and Tzanetakis, N (1997) In vitro study of lactobacillus species strains on bile tolerance and cholesterol removal. In: Lactic Acid Bacteria - Lactic 97. Caen: Presses universitaires de Caen.
Zhao Q, Liu Z, Xue Y, Wang J, Li H, Tang Q, Wang Y, Dong P, Xue C and Ds-echinoside A (2011) A new triterpene glycoside derived from sea cucumber, exhibits anti metastatic activity via the inhibition of NF-kB-dependent MMP-9 and VEGF expressions. Journal of Zhejiang University- Science 12 534-544
