A Study on Bacteriocin Produced from a Novel Strain of Lactobacillus crustorum F11 Isolated from Human Milk

Shweta Handa

Shweta Handa Microbiology Research Laboratory, Department of Basic Sciences, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, HP http://orcid.org/0000-0002-9248-9856

Keywords: Human milk, Lactobacillus crustorum, Bacteriocin, Antagonism

Abstract

Potential probiotic strain of Lactobacillus crustorum F11, isolated from Human milk was studied for the production of bacteriocin. L. crustorum F11 identified by morphological, and biochemical techniques followed by 16S rRNA sequencing,
NCBI accession number KT865221. Bacteriocin potential of L. crustorum F11, studied for the first time, was revealed by strong antagonism against food spoiling and pathogenic bacteria like Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogens, Clostridium perfringens, Leucononstoc mesenteroids and Bacillus cereus. Bacteriocin production was noticed in the late log and beginning of the stationary phase. Bacteriocin was sensitive to proteolytic enzymes like trypsin
and proteinase K, thus confirming its proteolytic nature. Bacteriocin activity identified in L. crustorum F11 has a bright prospective for use as food bio-preservative.