Photophysics and Rotational Dynamics of a Fluoroquinolone Drug Norfloxacin in Biomimetic Reverse Micellar Nanocavities

  • Saptarshi Mukherjee Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhauri, Bhopal 426 066, Madhya Pradesh
Keywords: Norfloxacin, Antibacterial Drug, AOT Reverse Micelle, Fluorescence Spectroscopy, Rotational Dynamics, Wobbling-in-Cone

Abstract

The present work describes the study of the photophysics and dynamics of a prospective anti-cancer antibacterial drug norfloxacin (NFX) within the micro-heterogeneous environment of Aerosol OT (AOT) reverse micelle (RM). The environment sensitive fluorescence spectroscopic behavior of NFX is found to exhibit a significant red-shift with increasing water pool size (w0=[water]/[surfactant]) of the RM. A prominent decrease of fluorescence anisotropy of NFX with increasing w0 demonstrates a relaxation of the motional restrictions imposed on the bound drug molecules with enhanced hydration.
These results are further substantiated from our time-resolved measurements. Study of the rotational dynamics of NFX within the RM as a function of w0 exhibits a typical biexponential decay behavior which has been adequately described on the basis of the wobbling-in-cone model. The rotational dynamical parameters are also calculated for wobbling motion of the drug within the RM as a function of w0.

Published
2016-10-19
Section
Research Papers